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Across most animal and human studies, two primary routes dominate [1]: Subcutaneous injection : Used in numerous rodent studies (typically 0.110 g/mouse/day) and in human trials (commonly 0.51 mg/day), especially in circadian and immune studies

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To lyse brain cell membranes, homogenates were mixed with vortex for 1 min on ice three times with 10 min intervals